MCB 4211 Basic Immunology
Tuesday/Thursday 2pm BPB 131
Lecture 4 Slide deck 9/10_post class
Lecture 5 Sept 15 as delivered
Link to PDF version of textbook for purchase: https://digitaltextbookhome.com/products/janeways-immunobiology-9th-edition-by-kenneth-murphy-9780815345053?_pos=1&_sid=532e40521&_ss=r
Required primary literature
Primary literature references from the scientific literature and their web links
- Kohler, G. and Milstein, C., Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, 1975. 256: p. 495-7. https://lyneslab.uconn.edu/wp-content/uploads/sites/2599/2020/09/Kohler-and-Milstein-.pdf
- Rennard, B.O., et al., Chicken soup inhibits neutrophil chemotaxis in vitro. Chest, 2000. 118(4): p. 1150-7. Download PDF
- Youn, J., et al., Metallothionein suppresses collagen-induced arthritis via induction of TGF-beta and down-regulation of proinflammatory mediators. Clin Exp Immunol, 2002. 129(2): p. 232-9. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906452/
- Dolk, E., M. van der Vaart, et al. (2005). “Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo.” Appl Environ Microbiol, 71(1): 442-50. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544197/pdf/0258-04.pdf
- 5.a. Siddle, H. V., A. Kreiss, M. D. Eldridge, E. Noonan, C. J. Clarke, S. Pyecroft, G. M. Woods, and K. Belov. 2007. Transmission of a fatal clonal tumor by biting occurs due to depleted MHC diversity in a threatened carnivorous marsupial. Proc Natl Acad Sci U S A 104:16221. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1999395&blobtype=pdf
- 5.b. https://www.nature.com/news/tasmanian-devils-show-signs-of-resistance-to-devastating-facial-cancer-1.20508
Optional (added value) Readings
- Hookworm mediated immune suppression and allergy/asthma treatment. http://www.nytimes.com/2008/07/01/health/research/01prof.html?_r=3&pa gewanted=1&sq&st=nyt&scp=2
- Lack of connection between vaccination and autism, and recent connections of autism to specific non-coding RNAs http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0003140
- http://the-scientist.com/2012/04/04/multiple-strikes-against-autism/
- autologous stem cells are rejected
- http://www.nytimes.com/2011/05/14/science/14stem.html?_r=2
- http://www.nature.com/nature/journal/vnfv/ncurrent/pdf/nature10135.pdf
- Social/Legal issues in Genetics and Immunology
- Vaccination programs and international policy http://www.ibtimes.com/articles/363531/20120716/pakistan-taliban-polio-vaccinations.htm
- Ferret Study Reinforces Role of Aerosols in SARS-CoV-2 Spread https://www.the-scientist.com/news-opinion/ferret-study-reinforces-role-of-aerosols-in-sars-cov-2-spread-68094
Vaccination in the US
- HIV prevention pill “Truvada” http://www.npr.org/blogs/health/2012/07/17/156868446/deciding-on-truvada-who-should-take-new-hiv-prevention-pill
- CAR-T cancer immunotherapies.
Ruella, M., and June, C. (2018) Predicting Dangerous Rides in CAR T Cells: Bridging the Gap between Mice and Humans. Mol. Ther. 26(6) 1401-1403. https://doi.org/10.1016/j.ymthe.2018.05.005
https://www.mdanderson.org/publications/cancerwise/2018/02/car-t-cell-therapy–9-things-to-know.html
- Environmental toxicants and immune disease
Kreitinger JM, Beamer CA, Shepherd DM. Environmental Immunology: Lessons learned from exposure to a select panel of immunotoxicants. Journal of immunology (Baltimore, Md : 1950). 2016;196(8):3217-3225. doi:10.4049/jimmunol.1502149. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824550/
Mouse as a model system:
Genomic responses in mouse models poorly mimic human inflammatory diseases
Immunological Differences Between Lab Mice and Wild Mice
The comparative immunology of wild and laboratory mice, Mus musculus domesticus
HIV Links:
HIV Replication 3D Medical Animation
https://www.biointeractive.org/classroom-resources/hiv-life-cycle
http://www.rkm.com.au/VIRUS/HIV/HIV-animation.html
Guidelines for the Use of Antiretroviral Agents in Adults and Adolescents with HIV
Accelerating HIV-1 Vaccine Efficacy Trials
Malignant Transformation of Hymenolepis nana in a Human Host
First New HIV Vaccine Efficacy Study in Seven Years Has Begun
Trispecific broadly neutralizing HIV antibodies mediate potent SHIV protection in macaques
HIV AIDS Replication Animation Video
HIV Life Cycle | HHMI BioInteractive Video
https://www.preventionaccess.org/consensus
CRISPR-Edited Stem Cells in a Patient with HIV and Acute Lymphocytic Leukemia
Antibody/Antigen:
Scientists hope animals’ immune systems will fight C. difficile
The genome sequence of Atlantic cod reveals a unique immune system
The Bursa of Fabricius: A Central Issue
https://www.stemcell.com/technical-resources/area-of-interest/immunology-research.html
Generation of Antibody Diversity through Gene Rearrangement and Somatic Hypermutation
The Scientists:
Karl Landsteiner- Nobel Prize Speech
George Williams, theorist on evolution
Genetics:
An integrated map of genetic variation from 1,092 human genomes
Pathogens/Vaccines:
Protein key for multiple malaria vaccine
First Results of Phase 3 Trial of RTS,S/AS01 Malaria Vaccine in African Children
Flu breakthrough promises a vaccine to kill all strains
What Will the Next Influenza Pandemic Look Like?
Eyam village and the Great Plague
- http://lynes.uconn.edu/Lynes_Lab/MCB4211_files/FLUCELVAX.pdf
- http://lynes.uconn.edu/Lynes_Lab/MCB4211_files/FLUZONEHD.pdf
China has withheld samples of a dangerous flu virus
Some website addresses of interest to immunologists: Immunology techniques biological materials, and instrumentation websites
Introduction to Flow Cytometry (video)
http://flowcyt.cyto.purdue.edu PUCL is the leading cytometry site since 1993
http://www.perkinelmer.com/lab-solutions {a manufacturers guide to immunoassays}
http://www.bdbiosciences.com/us/home/ {a home page for a flow cytometer
manufacturer}
http://www.atcc.org/ {American Type Culture Collection; a source of cell lines and genetic resources}
http://www.jax.org {a resource of inbred mouse strains, transgenic and
knockout mice}
Cell Biology
Hematopoietic Stem and Immune Cell Progenitors
Human fibroblasts to multilineage blood progenitors
Snake blood induces heart growth
Change skin into blood with no stops
Monocyte and macrophage differentiation
Origins and functions of tissue macrophages
Interesting Videos
Membrane and its molecular interaction
Leukocyte extravasation with explanation
Mouse Farming
Mouse genetics concepts and applications
Leptin: a piece of obesity pie
Rapamycin extends lifespan in genetically heterogeneous mice
Humanized mice in drug testing
Interesting Links
Genetic Studies of the Etiology of Type 2 Diabetes in Pima Indians
Summary of Universal Flu Vaccine Clinical Trial
Putting us all at risk for Measles
Autism/vaccine debate continues despite data
Moratorium on gain of function research
Influenza vaccine alternatives
Immunodeficiency foundation website
Save the Tasmanian Devil Project
Neutrophils lead, T cells follow
{Zinkernagel and Doherty paper}
Clinical trial hookworm and MS
{GWAS paper}
Cleaning the porcine genome of viruses that prevent safe xenografting
New connections between the immune system and the brain
Immunology databases
http://www.ncbi.nlm.nih.gov/protein {a site that shows structural features of CD molecules}
http://rarediseases.info.nih.gov/ {the office of rare diseases at NIH; for autoimmune diseases like Lupus, arthritis}
Genome databases
http://www.informatics.jax.org {mouse genome information}
http://www.ncbi.nlm.nih.gov/omim {online mendelian inheritance in man}
Scientific journal websites
http://www.cell.com/ {the journal Cell}
http://journals.lww.com/jaids/pages/default.aspx {Journal of Acquired Immune Deficiency Syndromes}
http://www.jimmunol.org/ {The Journal of Immunology}
http://flowcyt.cyto.purdue.edu/flowcyt/websites/cytsites/sitesed.htm {listing of other scientific journal websites}
Scientific funding websites
http://www.projectreporter.nih.gov/reporter.cfm {a site from which to search for currently funded NIH grants}
http://www.nsf.gov/awardsearch/ {NSF awards data base}
Scientific document search engines
http://www.ncbi.nlm.nih.gov/pubmed/ {for searching scientific journal citations “PubMed/Medline”
http://toxnet.nlm.nih.gov/ {the National Library of Medicine’s Toxline database of toxic chemicals}
Scientific reagent and instrumentation companies:
http://www.bdbiosciences.com/reagents/#research {a supplier of monoclonal antibodies to human, mouse antigens and a flow cytometry company}
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_2421.pdf {BioRad fluorescent probe specta}